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Impact of methyl jasmonate on squalene biosynthesis in microalga Schizochytrium mangrovei

Paper ID Volume ID Publish Year Pages File Format Full-Text
35495 45092 2009 5 PDF Available
Title
Impact of methyl jasmonate on squalene biosynthesis in microalga Schizochytrium mangrovei
Abstract

Squalene is an effective antioxidant and a potential chemopreventive agent. In this work, the effect of methyl jasmonate (MJA) on squalene biosynthesis in microalga Schizochytrium mangrovei was investigated. The maximum squalene content (1.17 ± 0.06 mg/g cell dry weight, DW) reached during the next 3 h after MJA treatment (0.1 mM) at 48 h of cultivation, which was 60% higher than that of control. The activity of squalene synthase (SS) increased 2-fold over control at this point. The maximum cholesterol content of 0.45 ± 0.03 mg/g DW was reached at hour 51 when MJA concentration was 0.4 mM, whereas the squalene content was lower at this point. The observations suggested that the increased squalene content was resulted from an increased activity of SS. MJA could be used to regulate the key enzymes in squalene biosynthetic pathway for the increased production of this compound in thraustochytrids. This research also provided novel information on the stimulation effect of methyl jasmonate on the biosynthesis of essential intermediate involved in the primary metabolism in microorganism.

Keywords
Methyl jasmonate; Squalene synthase; Schizochytrium mangrovei; Squalene; Cholesterol
First Page Preview
Impact of methyl jasmonate on squalene biosynthesis in microalga Schizochytrium mangrovei
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 8, August 2009, Pages 923–927
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering