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Combination of two lipases more efficiently catalyzes methanolysis of soybean oil for biodiesel production in aqueous medium

Paper ID Volume ID Publish Year Pages File Format Full-Text
35512 45093 2010 6 PDF Available
Title
Combination of two lipases more efficiently catalyzes methanolysis of soybean oil for biodiesel production in aqueous medium
Abstract

A combination of two lipases was employed to catalyze methanolysis of soybean oil in aqueous medium for biodiesel production. The two lipase genes were cloned from fungal strains Rhizomucor miehei and Penicillium cyclopium, and each expressed successfully in Pichia pastoris. Activities of the 1,3-specific lipase from R. miehei (termed RML) and the non-specific mono- and diacylglycerol lipase from P. cyclopium (termed MDL) were 550 U and 1545 U per ml respectively, and enzymatic properties of these supernatant of fermentation broth (liquid lipase) were stable at 4 °C for >3 months. Under optimized conditions, the ratio of biodiesel conversion after 12 h at 30 °C, using RML alone, was 68.5%. When RML was assisted by addition of MDL, biodiesel conversion ratio was increased to >95% under the same reaction conditions. The results suggested that combination of lipases with different specificity, for enzymatic conversion of more complex lipid substrates, is a potentially useful strategy for biodiesel production.

Keywords
FAME, fatty acid methyl ester(s) (termed “biodiesel”); TG, triglyceride; DG, diaglyceride; MG, monoglyceride; FFA, free fatty acid; RML, Rhizomucor miehei lipase; MDL, mono- and diacylglycerol lipase from Penicillium cyclopium; TLC, thin layer chromatogra
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Combination of two lipases more efficiently catalyzes methanolysis of soybean oil for biodiesel production in aqueous medium
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 10, October 2010, Pages 1677–1682
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us