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Production of novel halo-alkali-thermo-stable xylanase by a newly isolated moderately halophilic and alkali-tolerant Gracilibacillus sp. TSCPVG

Paper ID Volume ID Publish Year Pages File Format Full-Text
35519 45093 2010 8 PDF Available
Title
Production of novel halo-alkali-thermo-stable xylanase by a newly isolated moderately halophilic and alkali-tolerant Gracilibacillus sp. TSCPVG
Abstract

An aerobic xylanolytic Gracilibacillus sp. TSCPVG growing at moderate to extreme salinity (1–30%) and neutral to alkaline pH (6.5–10.5) was isolated from the salt fields near Sambhar district of Rajasthan, India. β-xylanase (18.44 U/ml) and β-xylosidase (1.01 U/ml) were produced in 60 h in the GSL-2 mineral base medium with additions of (in g/l) Birchwood xylan (7.5), yeast extract (10.0), tryptone (8.0), proline (2.0), thiamine (2.0), Tween-40 (2.0) and NaCl (35) at pH 7.5, 30 °C and 180 rpm. The β-xylanase was active within a broad salinity range (0–30% NaCl), pH (5.0–10.5) and temperature (50–70 °C). It exhibited maximal activity with 3.5% NaCl, pH 7.5 at 60 °C. It was extremely halotolerant retaining more than 80% of activity at 0 and 30% NaCl and alkali-tolerant retaining 76% of activity at pH 10.5. The acetone precipitated xylanase was highly stable (100%) at variable salinities of 0–30% NaCl, pH of 5.0–10.5 and temperatures of 0–60 °C for 48 h. HPLC analysis showed xylose, arabinose and xylooligosaccharides as hydrolysis products of xylan. This is the first report on hemi-cellulose degrading halo-alkali-thermotolerant enzyme from a moderately halophilic Gram-positive Gracilibacillus species.

Keywords
Gracilibacillus sp. TSCPVG; Moderate halophile; Alkali-tolerant; Halo-thermo-alkali-stable; β-xylanase; Xylooligosaccharides
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Production of novel halo-alkali-thermo-stable xylanase by a newly isolated moderately halophilic and alkali-tolerant Gracilibacillus sp. TSCPVG
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 10, October 2010, Pages 1730–1737
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us