fulltext.study @t Gmail

The effects of elevated process temperature on the protein carbonyls in the filamentous fungus, Aspergillus niger B1-D

Paper ID Volume ID Publish Year Pages File Format Full-Text
35536 45094 2008 5 PDF Available
Title
The effects of elevated process temperature on the protein carbonyls in the filamentous fungus, Aspergillus niger B1-D
Abstract

It is now accepted that heat treatment can lead to oxidative stress events in microorganisms, but there are few detailed studies on protein oxidation and consequent recycling/elimination of oxidatively damaged proteins following such heat treatment. In batch cultures of a filamentous fungus, Aspergillus niger B1-D, raising culture temperature from 25 °C to 30 °C and 35 °C led to a general enhancement of intracellular metabolism, a higher specific growth rate, increased consumption of carbon and nitrogen sources, and raised intracellular ATP content in the exponential phase. By contrast, there was a transient accumulation of protein carbonyls, a widely used biomarker of protein oxidation, following such temperature increases, which could indicate that cellular antioxidant defences were being temporarily overwhelmed under these circumstances, despite the fact that enhanced activities in antioxidant enzyme activities have been reported in microorganisms during such heat treatment. Protein carbonyls can only be removed by proteolysis. The intracellular proteolytic activity in batch cultures of A. niger was found generally enhanced by temperature elevation, suggesting the role of proteolytic activity in protein quality control during heat treatment was dependant on the culture temperature.

Keywords
Protein oxidation; Proteolytic activity; Filamentous fungi; Batch culture
First Page Preview
The effects of elevated process temperature on the protein carbonyls in the filamentous fungus, Aspergillus niger B1-D
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 8, August 2008, Pages 877–881
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us