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Co-production of a whole cellulase system in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
3554 174 2012 7 PDF Available
Title
Co-production of a whole cellulase system in Escherichia coli
Abstract

Usually, glucose production from cellulose hydrolysis involves synergistic actions by exoglucanase, endoglucanase and β-glucosidase. To co-produce a whole cellulase system in Escherichia coli, a multi-promoter construct and a multi-cistronic construct harboring an exoglucanase (CbhA), an endoglucanase (CenA) and a β-glucosidase (BGL) were built herein. Synergistic effect of three enzymes was also investigated. The results showed synergism of CenA and BGL played a critical role in degrading filter paper to glucose. Compared with monocistronic constructs, multi-promoter and multi-cistronic constructs harboring three enzymes showed significantly higher glucose yield. The specific activity of multi-promoter construct was 22.5-fold and 6.9-fold higher than CbhA and CenA, respectively and that of multi-cistronic construct was 33.6-fold and 10.3-fold higher than CbhA and CenA, respectively. In conclusion, a whole cellulase system could be co-produced in E. coli for converting cellulose to glucose. Moreover, the strategy could be easily applied to other cellulolytic enzymes, as well as for other multi-enzymatic systems.

► A multi-promoter construct was built to co-express a whole cellulase system. ► A multi-cistronic construct was built to co-express a whole cellulase system. ► Synergistic effect of cellulases expressed in the work was investigated. ► Significantly higher glucose was produced with co-expression strategy.

Keywords
Cellulase; Co-expression; Escherichia coli; Enzyme production; Enzymes; Cellulose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 69, 15 December 2012, Pages 204–210
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us