fulltext.study @t Gmail

Production of non-monosaccharide and high-purity galactooligosaccharides by immobilized enzyme catalysis and fermentation with immobilized yeast cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
35540 45094 2008 4 PDF Available
Title
Production of non-monosaccharide and high-purity galactooligosaccharides by immobilized enzyme catalysis and fermentation with immobilized yeast cells
Abstract

A process for producing non-monosaccharide and high-purity galactooligosaccharides (GOS) was proposed. GOS were produced from lactose by Penicillium expansum F3 β-galactosidase immobilized in calcium alginate. The effects of pH, temperature and lactose concentration on GOS production were investigated. A purity of 28.7% (w/w) GOS was obtained from 380 g/L lactose solution at pH 5.4 and 50 °C. The immobilized enzyme was used for repeated GOS synthesis and showed good operational stability. Digestible sugars in the GOS could be depleted after fermentation with Saccharomyces cerevisiae L1 or Kluyveromyces lactis L3 entrapped in the calcium alginate. Purity greater than 37% with yields greater than 27% of non-monosaccharide GOS were maintained by S. cerevisiae L1 for 19 batches and purity greater than 97% with a yield greater than 20% of high-purity GOS was produced using K. lactis L3 for two batches.

Keywords
Galactooligosaccharides; Penicillium expansum; β-Galactosidase; Yeast fermentation; Saccharomyces cerevisiae; Kluyveromyces lactis
First Page Preview
Production of non-monosaccharide and high-purity galactooligosaccharides by immobilized enzyme catalysis and fermentation with immobilized yeast cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 8, August 2008, Pages 896–899
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering