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Fructose production by chicory inulin enzymatic hydrolysis: A kinetic study and reaction mechanism

Paper ID Volume ID Publish Year Pages File Format Full-Text
35556 45095 2009 5 PDF Available
Title
Fructose production by chicory inulin enzymatic hydrolysis: A kinetic study and reaction mechanism
Abstract

In this paper, a reaction scheme for fructose production by inulin enzymatic hydrolysis is proposed, taking into account the possibility of dealing with a mixture of enzymes acting on a mixture of polymers as substrate. The scheme is subsequently simplified to obtain a stoichiometric relationship between the fructose product and the reacted substrate. The former may be measured by HPLC, while the latter is the subject of kinetic investigations. Our proposed kinetic model is defined within temperature and substrate concentration ranges of industrial interest (40–60 °C and 3–60 g/L, respectively). Some assumptions were made in order to simplify the model, which is based on a minimum number of parameters. These hypotheses were always specified and assumed only on the basis of convenience and rational consideration. Eventually, the kinetic model was successfully validated by comparison with a vast set of experimental results.

Keywords
Inulinase; Fructose; Kinetic model; Bio-catalysis; Reaction mechanism; Depolymerization
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Fructose production by chicory inulin enzymatic hydrolysis: A kinetic study and reaction mechanism
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 4, April 2009, Pages 466–470
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us