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Partitioning of protease from stomach of albacore tuna (Thunnus alalunga) by aqueous two-phase systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
35557 45095 2009 6 PDF Available
Title
Partitioning of protease from stomach of albacore tuna (Thunnus alalunga) by aqueous two-phase systems
Abstract

Partitioning of protease from stomach of albacore tuna using an aqueous two-phase system (ATPS) was investigated. The best ATPS conditions for protease partitioning from stomach extract (SE) and acidified counterpart (ASE) were 25% PEG1000–20% MgSO4 and 15% PEG2000–15% MgSO4, which increased the purity by 7.2-fold and 2.4-fold with the recovered activity of 85.7% and 89.1%, respectively. Electrophoretic study revealed that SE had a major protein with a molecular weight (MW) of 40.6 kDa, while protein with MW of 32.7 kDa was predominant in ASE and ATPS fractions. Pepsinogen in SE might be activated to pepsin by acidification and partitioning process. SE was quite stable at 0 and 4 °C up to 14 days. The loss in protease activity in ASE and selected ATPS fractions was more pronounced when storage time and temperature increased. Therefore, ATPS can be effectively used to recover and purify protease from albacore tuna stomach.

Keywords
Stomach; Tuna; Albacore; Aqueous two-phase system; Protease; Partitioning
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Partitioning of protease from stomach of albacore tuna (Thunnus alalunga) by aqueous two-phase systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 4, April 2009, Pages 471–476
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us