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Size exclusion chromatography of zein and xanthophylls: Optimization of operating parameters

Paper ID Volume ID Publish Year Pages File Format Full-Text
35559 45095 2009 5 PDF Available
Title
Size exclusion chromatography of zein and xanthophylls: Optimization of operating parameters
Abstract

Ethanol-soluble components of corn (zein and xanthophylls) were separated and purified by size exclusion chromatography. Aqueous ethanol was used as the solvent for the entire process from extraction through chromatography. The effect of operating and design parameters, such as temperature, flow rate, loading mass, loading volume, column height and diameter, on productivity and resolution of the components was studied. Using a one-variable-at-a-time (OVAT) approach with 1 cm × 60 cm columns, optimum conditions were determined to be 40 °C temperature, 0.25 mL/min flow rate, volume loading of 22 mL corn extract, mass loading at 70 g/L of corn extract. All components could be resolved with base line separation with a 240 cm column length. Column diameter did not affect separation, implying linear scalability with constant flow distribution.

Keywords
Process chromatography; Optimization; SEC; Corn; Zein; Xanthophylls
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Size exclusion chromatography of zein and xanthophylls: Optimization of operating parameters
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 4, April 2009, Pages 481–485
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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