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Purification and identification of an angiotensin I-converting enzyme inhibitory peptide from fermented soybean extract

Paper ID Volume ID Publish Year Pages File Format Full-Text
35561 45095 2009 4 PDF Available
Title
Purification and identification of an angiotensin I-converting enzyme inhibitory peptide from fermented soybean extract
Abstract

Angiotensin I-converting enzyme (ACE), a dipeptidyl carboxypeptidase, plays an important physiological role in regulating blood pressure. ACE-inhibitory peptides derived from food proteins have potential pharmaceutical and human health uses. In this study, we prepared a fermented soybean extract (FSE) through a rapid fermentation at an elevated temperature to accelerate proteolytic hydrolysis and described purification procedures to discover potent ACE-inhibitory peptides from FSE. After 3 days of aging, FSE exhibited ACE-inhibitory activity with an IC50 value of 1.46 mg/mL. Purification of novel ACE-inhibitory peptides was carried out using ultrafiltration and consecutive chromatographic methods. A novel ACE-inhibitory peptide, with 66-fold increase in ACE-inhibitory activity compared to that of FSE, was isolated from FSE through a five-step purification procedure. The amino acid sequence of the purified ACE-inhibitory peptides was determined to be Leu-Val-Gln-Gly-Ser by Edman degradation method, and its IC50 value was 22 μg/mL (43.7 μM).

Keywords
Soybean extract; Fermentation; ACE-inhibitory peptide; Bioactive peptide; Antihypertensive
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Purification and identification of an angiotensin I-converting enzyme inhibitory peptide from fermented soybean extract
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 4, April 2009, Pages 490–493
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us