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Isolation and preliminary characterization of a nonbacteriocin antimicrobial compound from Weissella paramesenteroides DFR-8 isolated from cucumber (Cucumis sativus)

Paper ID Volume ID Publish Year Pages File Format Full-Text
35563 45095 2009 5 PDF Available
Title
Isolation and preliminary characterization of a nonbacteriocin antimicrobial compound from Weissella paramesenteroides DFR-8 isolated from cucumber (Cucumis sativus)
Abstract

A non-proteinaceous antimicrobial substance (nonbacteriocin) produced by Weissella paramesenteroides DFR-8, an isolate from cucumber (Cucumis sativus), was purified using cell adsorption–desorption and gel permeation chromatography methods. A single peak observed in the purity check by analytical RP-HPLC strongly indicates the homogeneity of the nonbacteriocin preparation. The active substance is insensitive to proteolytic enzymes, lipase, amylase and catalase and shows a broad spectrum of activity towards food-borne/spoilage pathogens including Gram-negative organisms. The non-proteinaceous antimicrobial molecule has a molecular mass ∼2.5 kDa and is thermostable up to 121 °C at pH ∼4.0. A central composite rotatable design (CCRD) was employed to study the interactive effect of temperature and pH on antimicrobial activity and an equation was developed to deduce the residual activity of inhibitory substance under any conditions of temperature and pH within the experimental domain. The broad inhibitory spectrum and heat stability of the antimicrobial substance advocates its application as food-biopreservative.

Keywords
LAB; Nonbacteriocin; Biopreservation; Food-borne pathogens; Antimicrobials; RSM
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Isolation and preliminary characterization of a nonbacteriocin antimicrobial compound from Weissella paramesenteroides DFR-8 isolated from cucumber (Cucumis sativus)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 4, April 2009, Pages 499–503
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us