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Modulation of yeast hexokinase on bio-inspired membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
3557 174 2012 6 PDF Available
Title
Modulation of yeast hexokinase on bio-inspired membranes
Abstract

Hexokinase (HK) is the first enzyme of the glycolytic pathway and is known to modulate its own activity by binding to the mitochondrial membrane. In this study, the enzymatic activity of HK was measured in the presence of various liposomes. The positively charged liposome with an appropriate charge density was found to increase the HK activity. The HK activity was enhanced 1.5-fold in the presence of 5 mol% didodecyldimethylammonium bromide (DDAB) and 1.8-fold with 5–10 mol% 3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol hydrochloride (DC-Cholesterol) on the POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocoline) liposome. Analysis of (i) HK binding onto liposome, (ii) intrinsic Trp fluorescence, and (iii) circular dichroism of HK suggested that the HK activity was enhanced on positively charged microdomain because of its slight conformational change through the electrostatic and hydrophobic interactions.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlight► The positively charged liposome enhanced the enzymatic activity of hexokinase (HK). ► HK activity was enhanced 1.8-fold on liposome with positively-charged microdomain. ► HK activity was enhanced because of its slight conformational change on the liposome.

Keywords
Membranome; Membrane stress biotechnology; Liposome; Hexokinase; Positive-charge
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Modulation of yeast hexokinase on bio-inspired membranes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 69, 15 December 2012, Pages 138–143
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us