fulltext.study @t Gmail

Purification of an α-amylase inhibitor in a polyethylene glycol/fructose-1,6-bisphosphate trisodium salt aqueous two-phase system

Paper ID Volume ID Publish Year Pages File Format Full-Text
35576 45096 2008 4 PDF Available
Title
Purification of an α-amylase inhibitor in a polyethylene glycol/fructose-1,6-bisphosphate trisodium salt aqueous two-phase system
Abstract

A novel aqueous two-phase system of polyethylene glycol (PEG)/fructose-1,6-bisphosphate (FBP) trisodium salt was employed to purify an α-amylase inhibitor (α-AI) from wheat flour after failure in use of the conventional aqueous two-phase systems of PEG/(NH4)2SO4 and PEG/dextran (DEX). Phase compositions including PEG molecular weight and concentration of PEG, fructose-1,6-bisphosphate trisodium salt concentration, as well as pH of the system affected the partition of α-AI. The inhibitor was obtained with a purification factor of 3.2 and recovery of 79% by using 11.7% (w/w) of PEG2000 and 19% (w/w) of fructose-1,6-bisphosphate trisodium salt at pH 7.0 in the top phase.

Keywords
α-Amylase inhibitor; Aqueous two-phase system; Fructose-1,6-bisphosphate-trisodium salt; PEG; Purification
First Page Preview
Purification of an α-amylase inhibitor in a polyethylene glycol/fructose-1,6-bisphosphate trisodium salt aqueous two-phase system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 7, July 2008, Pages 765–768
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us