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Optimized extraction of a lectin from Crataeva tapia bark using AOT in isooctane reversed micelles

Paper ID Volume ID Publish Year Pages File Format Full-Text
35579 45096 2008 4 PDF Available
Title
Optimized extraction of a lectin from Crataeva tapia bark using AOT in isooctane reversed micelles
Abstract

Crataeva tapia bark lectin was extracted from a crude extract into a reversed micelle phase of the anionic surfactant AOT in isooctane and back-extracted, to a final aqueous phase by addition of butanol. The effects of pH, ionic strength and surfactant concentration on the protein transfer process from the aqueous to the organic phase were characterized, being the best results obtained after 5 min of contact, under agitation, between the two phases, at pH 5.5 (10 mM citrate-phosphate buffer), 30 mM NaCl, and 5 mM AOT. Recovery to a new aqueous phase was performed with 5 min of contact, under agitation, 10 mM citrate-phosphate buffer at pH 5.5, 500 mM KCl and 5% of butanol. The overall yield obtained for the process was 80% for lectin activity and 56% for protein recovery. The efficiency of the process was confirmed by SDS-PAGE analysis.

Keywords
Liquid–liquid extraction; Reversed micelles; Sodium di(2-ethylhexyl)sulfosuccinate (AOT); Purification; Lectin; Crataeva tapia
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Optimized extraction of a lectin from Crataeva tapia bark using AOT in isooctane reversed micelles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 7, July 2008, Pages 779–782
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us