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The release of hepatitis B core antigen from Escherichia coli by batch mode bead milling

Paper ID Volume ID Publish Year Pages File Format Full-Text
35594 45097 2008 7 PDF Available
Title
The release of hepatitis B core antigen from Escherichia coli by batch mode bead milling
Abstract

The performance of a batch model bead mill on the release of hepatitis B core antigen (HBcAg) from Escherichia coli was investigated in this study. The operating parameters examined were impeller tip speed (8–14 m/s), biomass concentration [5–20% (w/v)] and bead loading [65–80% (v/v)]. The highest yield (24.3 mg/g cell) and rate constant (0.471 l/min) of HBcAg release were achieved at impeller tip speed of 14 m/s. However, the high-shear stress under these operating conditions caused damage of the HBcAg. The highest yield (22.7 mg/g cell) and rate constant (0.344 l/min) of HBcAg release were observed at biomass concentration of 20% (w/v). There was no significant effect of bead loading on the performance of bead milling being observed. In conclusion, the optimal operating condition for the release of HBcAg was at bead loading of 75% (v/v), biomass concentration of 20% (w/v) and impeller tip speed of 10 m/s.

Keywords
Bead mill; Batch mode; Cell disruption; Hepatitis B core antigen; Downstream process; Escherichia coli
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The release of hepatitis B core antigen from Escherichia coli by batch mode bead milling
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 2, February 2008, Pages 206–212
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us