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Solid-state fermentation for ‘whole-cell synthetic lipase’ production from Rhizopus chinensis and identification of the functional enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
35596 45097 2008 6 PDF Available
Title
Solid-state fermentation for ‘whole-cell synthetic lipase’ production from Rhizopus chinensis and identification of the functional enzyme
Abstract

Major process parameters affecting the production of whole-cell synthetic lipase (WCSL) by Rhizopus chinensis in solid-state fermentation were optimized based on synthetic activity. A combined substrate of wheat flour with wheat bran (3/2, w/w) supported both good biomass growth and enzyme production. The maximum synthetic activity of 24,447 U/kg substrate was reached by selecting a moisture content of 70%, initial pH of 6.5, supplementation of peptone (2%, w/w) as additional nitrogen source and olive oil (2%, v/w) as inducer, after 72 h of incubation. This production represented a 15.27-fold increase of lipase synthetic activity compared to the initial medium. The synthetic lipase was purified by ammonium sulfate precipitation, hydrophobic interaction and gel filtration to homogeneity, with yield and purification fold of 2.6% and 55.2, respectively, and the molecular weight was estimated to be 60 kDa by SDS-PAGE. Validation of functional enzyme was carried out by catalyzing the synthesis of ethyl flavor esters, and the result showed that conversion of ethyl octanoate and ethyl caprate reached 95%.

Keywords
Whole-cell lipase; Synthetic activity; Solid-state fermentation; Rhizopus chinensis; Ethyl ester
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Solid-state fermentation for ‘whole-cell synthetic lipase’ production from Rhizopus chinensis and identification of the functional enzyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 2, February 2008, Pages 219–224
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us