fulltext.study @t Gmail

Biosynthesis of ethyl butyrate by immobilized recombinant Rhizopus oryzae lipase expressed in Pichia pastoris

Paper ID Volume ID Publish Year Pages File Format Full-Text
3560 175 2012 9 PDF Available
Title
Biosynthesis of ethyl butyrate by immobilized recombinant Rhizopus oryzae lipase expressed in Pichia pastoris
Abstract

A recombinant Rhizopus oryzae lipase expressed in Pichia pastoris has been immobilized in three different kinds of supports: EP100, Eupergit®CM and Octadecyl-Sepabeads. These immobilized derivatives have been used to catalyze the synthesis of ethyl butyrate. The effect of different parameters on initial reaction rate and yield has been studied for each biocatalyst. The variables selected for the study were: temperature, water content, shaking speed, enzyme loading, type of solvent and substrates molar ratio. The results showed the EP100 derivative as the best choice in terms of initial rate and yield but, when the residual activity as well as the reutilization capacity was studied, Octadecyl-Sepabeads exhibits the highest stability, so it was finally chosen as the best biocatalyst.

► Recombinant Rhizopus oryzae lipase (rROL) catalyzes the synthesis of ethyl butyrate. ► Determination of the influence of different parameters on synthesis reaction. ► rROL immobilized on EP100 showed the highest initial synthesis rate. ► rROL immobilized on Sepabeads showed the best operational stability.

Keywords
Pichia pastoris; Rhizopus oryzae; Recombinant lipase; Flavor synthesis; Ethyl butyrate; Immobilized lipase
First Page Preview
Biosynthesis of ethyl butyrate by immobilized recombinant Rhizopus oryzae lipase expressed in Pichia pastoris
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 65, 15 June 2012, Pages 1–9
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering