fulltext.study @t Gmail

Purification, structural analysis and hydroxyl radical-scavenging capacity of a polysaccharide from the fruiting bodies of Russula virescens

Paper ID Volume ID Publish Year Pages File Format Full-Text
35605 45098 2010 6 PDF Available
Title
Purification, structural analysis and hydroxyl radical-scavenging capacity of a polysaccharide from the fruiting bodies of Russula virescens
Abstract

In this study, one water-soluble polysaccharide (RVP) was isolated from the fruiting bodies of Russula virescens. Its molecular weight (Mw) was ∼3.9 ×104 Da, as determined by high-performance size-exclusion chromatography (HPSEC). The RVP was composed of d-galactose and d-mannose in a ratio of 2:1, as detected by gas chromatography (GC). The structural features of RVP were investigated by partial hydrolysis with acid, periodate oxidation and Smith degradation, acetylation, methylation analysis, and NMR spectroscopy (1H, 13C). The results revealed that RVP had a backbone consisting of (1 → 6)-linked-α-d-galactopyranosyl and (1 → 2, 6)-linked-α-d-galactopyranosyl residues that terminated in a single non-reducing terminal (1 → )-α-d-mannopyranosyl residue at the O-2 position of each (1 → 2, 6)-linked-α-d-galactopyranosyl residues along the main chain in the ratio of 1:1:1. Preliminary, in vitro antioxidant activity tests showed that RVP could dose-dependently enhance the scavenging effect on hydroxyl radicals at concentrations from 20 to 160 μg/ml. These results indicated that RVP was mainly composed of water-soluble galactomannan, and it had a significant effect on scavenging of hydroxyl radicals in vitro.

Keywords
Russula virescens; Polysaccharide; Structural analysis; Antioxidant activity
First Page Preview
Purification, structural analysis and hydroxyl radical-scavenging capacity of a polysaccharide from the fruiting bodies of Russula virescens
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 6, June 2010, Pages 874–879
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering