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A highly selective ginsenoside Rb1-hydrolyzing β-d-glucosidase from Cladosporium fulvum

Paper ID Volume ID Publish Year Pages File Format Full-Text
35609 45098 2010 7 PDF Available
Title
A highly selective ginsenoside Rb1-hydrolyzing β-d-glucosidase from Cladosporium fulvum
Abstract

G-I, a highly selective β-glucosidase, was purified from phytopathogenic fungus Cladosporium fulvum (syn. Fulvia fulva). G-I was a monomer with native molecular weight of 85 kDa and pI value of 4.2. The maximal activity to p-nitrophenyl-β-d-glucopyranoside (pNPG) occurred at pH 6.0 and 45 °C at which the Km against pNPG was 0.18 mM and Vmax was 46.7 μmol nitrophenol/min/mg. G-I was highly stable within pH 4.0–11.0 and below 40 °C. It was inhibited by Co2+, Cu2+ and Zn2+ (50 mM), but showed resistance to sodium dodecyl sulfonate (SDS, 250 mM). G-I was highly active against β-linked disaccharide cellobiose, gentiobiose and sophorose, but exhibited very low activities against other aryl-glycosides, methyl-α-glycosides and disaccharides trehalose and sucrose. Moreover, G-I specifically hydrolyzed β-(1 → 6)-glucosidic linkage at the C-20 site of ginsenoside Rb1 to produce ginsenoside Rd, without attack on other β-d-glucosidic linkages. The oligopeptide fragments of G-I were sequenced by nanoESI-MS/MS and showed similarity to the sequences from the glycoside hydrolase family 3. G-I is different to G-II (a glycosidase previously purified from the same fungus) in composition and molecular weight. It shows more stable and higher selectivity than G-II.

Keywords
β-Glucosidase; Cladosporium fulvum (syn. Fulvia fulva); Ginsenoside Rb1; Ginsenoside Rd; Biotransformation; Selectivity
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A highly selective ginsenoside Rb1-hydrolyzing β-d-glucosidase from Cladosporium fulvum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 6, June 2010, Pages 897–903
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us