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Biotransformation of procyanidins by a purified fungal dioxygenase: Identification and characterization of the products using mass spectrometry

Paper ID Volume ID Publish Year Pages File Format Full-Text
35610 45098 2010 10 PDF Available
Title
Biotransformation of procyanidins by a purified fungal dioxygenase: Identification and characterization of the products using mass spectrometry
Abstract

Procyanidins commonly known as condensed tannins are a type of polyphenol with wide abundance naturally. They are commonly known as potent anti-oxidants with powerful free radical scavenging activity as well as anti-tumor-promoting activity. Little is known about the enzymatic mechanisms/pathways involved in the microbial biotransformation of these polyphenolic molecules. The extracellular enzyme, dioxygenase produced by Aspergillus fumigatus was used as in vitro tools to study the degradation pathway of a model procyanidin dimer, namely procyanidin B2. The enzyme was purified to homogeneity by a two step process of anion-exchange chromatography coupled with FPLC followed by gel-filtration chromatography coupled with HPLC and the molecular mass estimated. In addition, the different biotransformed products resulted from the dioxygenase action on PB2 were purified using Reversed-Phase-High Performance Liquid Chromatography prior to their identification and characterization by structural elucidation using Electrospray Ionization-Mass Spectrometry. Subsequently, the mechanism of dioxygenase action on procyanidin dimer was defined.

Keywords
Procyanidins; Aspergillus fumigatus; Dioxygenase; Purification; Reversed-Phase High Performance Liquid Chromatography; Electrospray Ionization-Mass Spectrometry
First Page Preview
Biotransformation of procyanidins by a purified fungal dioxygenase: Identification and characterization of the products using mass spectrometry
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 6, June 2010, Pages 904–913
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering