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Enzymatic production of bioactive peptides from sericin recovered from silk industry wastewater

Paper ID Volume ID Publish Year Pages File Format Full-Text
35629 45099 2008 8 PDF Available
Title
Enzymatic production of bioactive peptides from sericin recovered from silk industry wastewater
Abstract

Bioactive peptides were produced from sericin, a by-product from silk industry wastewater, by using Protease P. The optimal hydrolysis conditions (pH of 8.40, temperature of 43.97 °C and the enzyme/substrate ratio of 3:100) were estimated by using response surface methodology with a maximum degree of hydrolysis of 14.43% and the minimum IC50 value for the ferrous ion-chelating activity of 0.128 mg/mL. The freeze-dried sericin hydrolysate (SH), produced by using Protease P and under the above optimal conditions, contained 82% of crude protein, 13% of moisture and 5% of ash. The physiochemical analysis shows that the amino acids with hydroxyl groups (Ser and Thr) of SH accounted for 30% or so, and SH was a mixture of hydrophilic peptides mostly distributed among 250–4000 Da. SH exhibited excellent antioxidant activities in two measurements, including the ferrous ion-chelating activity and the reducing power. Moreover, SH also exhibited a notable tyrosinase-inhibitory effect in a dose-dependent manner. This study firstly showed an effective bioprocess for the production of sericin bioactive peptides, which may be used as valuable ingredients in the food, cosmetic and medicine industries.

Keywords
Sericin; Bioactive peptide; Response surface methodology; Hydrolysate; Antioxidant; Tyrosinase
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Enzymatic production of bioactive peptides from sericin recovered from silk industry wastewater
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 5, May 2008, Pages 480–487
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us