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A novel thermophilic anaerobic bacteria producing cyclodextrin glycosyltransferase

Paper ID Volume ID Publish Year Pages File Format Full-Text
35648 45100 2009 7 PDF Available
Title
A novel thermophilic anaerobic bacteria producing cyclodextrin glycosyltransferase
Abstract

A novel thermophilic anaerobic, rod-shaped, non-spore forming, gram positive bacterium was isolated from an oil field in Turkey, that produces cyclodextrin glycosyltransferase (CGTase) from starch. According to the some morphological, biochemical and 16S rRNA analysis, the strain belongs to the genus Thermoanaerobacter. The strain mainly utilizes starch and derivatives, glucose and fructose as carbon source between 45 and 75 °C, optimally at 65 °C. Optimum pH for growth is 7.5. 16S RNA studies indicate that the bacterium has a similarity of 98.3% to homoacetogenic Thermoanaerobacter kivui and the main fermentation product is acetic acid as in the case with homoacetogenic bacteria. The main difference between the bacterium and T. kivui concerns the utilization of starch. Based on the phylogenetic and biochemical analysis, it is proposed that the species are a new member of the genus Thermoanaerobacter. The strain has CGTase activity optimum at 80 °C and pH 7.0–8.0.

Keywords
Thermoanaerobacter; Thermophilic anaerobic; Cyclodextrin glycosyltransferase; Cyclodextrin; Soluble starch
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A novel thermophilic anaerobic bacteria producing cyclodextrin glycosyltransferase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 1, January 2009, Pages 36–42
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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