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Proteolytic activity in enzymatic extracts from Carica papaya L. cv. Maradol harvest by-products

Paper ID Volume ID Publish Year Pages File Format Full-Text
35653 45100 2009 6 PDF Available
Title
Proteolytic activity in enzymatic extracts from Carica papaya L. cv. Maradol harvest by-products
Abstract

Proteolytic activity and the cysteine protease profile were determined for enzymatic extracts (EE) from Carica papaya L. cv. Maradol harvest by-products (stems, unripe fruit, petioles and leaves). The proportion of each by-product type in the sampled plantation was calculated. Polypeptide bands were identified by SDS–PAGE for each EE and molecular weight calculated for the cysteine proteases. Leaf and fruit tissue had the highest protein contents of the by-products. Leaf tissue also produced the highest total EE yield. All the SDS–PAGE gels for the EE’s exhibited an approximately 23 kDa band probably corresponding to papain. The zymography profiles of the EE’s were similar, with bands at approximately >202.8, 76.8, 55.4 and 46.5 kDa. The fruit EE had the highest specific proteolytic activity and the leaf EE the lowest. Fruit and stem by-products are the most promising for proteolytic enzyme extraction.

Keywords
Carica papaya; Papaya by-products; Enzymatic extracts; Latex; Cysteine proteases; Zymography profiles
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Proteolytic activity in enzymatic extracts from Carica papaya L. cv. Maradol harvest by-products
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 1, January 2009, Pages 77–82
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us