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Lipase catalytic synthesis of diacylglycerol from tuna oil and its anti-obesity effect in C57BL/6J mice

Paper ID Volume ID Publish Year Pages File Format Full-Text
35679 45101 2010 6 PDF Available
Title
Lipase catalytic synthesis of diacylglycerol from tuna oil and its anti-obesity effect in C57BL/6J mice
Abstract

Diacylglycerol (DAG) production by glycerolysis of glycerol with tuna oil was performed using lipases from Rhizomucor miehei (Lipozyme RMIM) and Candida antarctica (Novozyme 435). Lipozyme RMIM caused a clear estrific positional specificity in HPLC analysis and then the Lipozyme RMIM was chosen for the production of DAG. Moreover, the reaction parameter for DAG synthesis was determined by measuring mole ratio of glycerol/tuna oil, amount of enzyme, temperature and water contents. The optimal mole ratio for glycerol/tuna oil was established to be 3:1. Optimal conditions of lipase, water and temperature were 10%, 10% and 35 °C, respectively. Therefore, we have synthesized DAG from tuna oil under these optimal conditions and investigated the effect of the synthesized DAG on body weight and plasma biochemical markers of obesity in C57BL/6J mice. The consumption of DAG diet has effectively lessened body weight gain and final plasma total cholesterol, triacylglycerol and glucose levels compared to the high triacylglycerol (TAG) group.

Keywords
Tuna oil; Diacylglycerol; Triacylglycerol; Obesity; Lipozyme RMIM; C57BL/6J
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Lipase catalytic synthesis of diacylglycerol from tuna oil and its anti-obesity effect in C57BL/6J mice
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 5, May 2010, Pages 738–743
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us