fulltext.study @t Gmail

Evaluation of recombinant phenylalanine dehydrogenase behavior in aqueous two-phase partitioning

Paper ID Volume ID Publish Year Pages File Format Full-Text
35698 45102 2007 6 PDF Available
Title
Evaluation of recombinant phenylalanine dehydrogenase behavior in aqueous two-phase partitioning
Abstract

Recombinant Bacillus sphaericus phenylalanine dehydrogenase (PheDH) partitioning was studied in polyethylene glycol (PEG) and ammonium sulfate aqueous two-phase systems (ATPS). The objectives of this work were to investigate influences; varying the molecular mass and concentration of PEG, pH, phase volume ratio (VR), tie-line length (TLL) and concentration of (NH4)2SO4 on the partition behavior of PheDH. It was revealed that the partitioning was not affected by VR, while PEG molecular mass and concentration and (NH4)2SO4 concentration had significant effects on enzyme partitioning. Longer TLL and higher pH resulted in better partitioning into the top phase. Under the most favorable partition conditions with 8.5% (w/w) PEG-6000, 17.5% (w/w) (NH4)2SO4 and VR = 0.25 at pH 8.0, partition coefficient (KE), recovery (R%), yield (Y%) and TLL were achieved 58.7%, 135%, 94.42% and 39.89% (w/w), respectively. Overall, the promising results obtained in this research indicated that the ATPS partitioning can be provided an efficient and powerful tool for recovery and purification of recombinant PheDH.

Keywords
Aqueous two-phase systems (ATPS); Ammonium sulfate; Phenylalanine dehydrogenase (PheDH); Partition; PEG-6000
First Page Preview
Evaluation of recombinant phenylalanine dehydrogenase behavior in aqueous two-phase partitioning
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 9, September 2007, Pages 1296–1301
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us