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Optimal continuous biosynthesis of hexyl laurate by a packed bed bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
35708 45102 2007 5 PDF Available
Title
Optimal continuous biosynthesis of hexyl laurate by a packed bed bioreactor
Abstract

Hexyl laurate, a medium-chain ester carried about fruity flavor, is primarily used in personal care formulations as an important emollient for cosmetic applications. On the basis of the hexyl laurate could be successfully synthesized by lipase within a batch system in our previous report. This study aimed to develop an optimal continuous procedure of lipase-catalyzed hexyl laurate synthesis in a packed-bed bioreactor to investigate the possibility of large-scale production further. The ability of lipase from Rhizomucor miehei (Lipozyme IM-77) to catalyze the direct-esterification of 1-hexanol and lauric acid in n-hexane was investigated. Response surface methodology (RSM) and 3-level-3-factor fractional factorial design were employed to evaluate the effects of synthesis parameters, such as reaction temperature (35–55 °C), mixture flow rate (1.5–4.5 mL/min) and substrate molar ratio 1-hexanol to lauric acid (1:1–1:3) on production rate (μmol/min) of hexyl laurate by direct-esterification. Based on the analysis of ridge max, the optimum synthesis conditions for hexyl laurate were as follows: 45 °C of reaction temperature, substrate molar ratio 1:2 and reaction flow rate 4.5 mL/min. The optimum predicted production rate was 435.6 ± 0.9 μmol/min and the actual value was 437.6 ± 0.4 μmol/min.

Keywords
Esterification; Hexyl laurate; Lipase; Packed bed reactor; Response surface methodology
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Optimal continuous biosynthesis of hexyl laurate by a packed bed bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 9, September 2007, Pages 1362–1366
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us