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Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards

Paper ID Volume ID Publish Year Pages File Format Full-Text
35712 45103 2007 6 PDF Available
Title
Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards
Abstract

Response surface methodology (RSM) was used to optimize the hydrolysis conditions (temperature, pH and Alcalase® 2,4L concentration), in order to obtain the hydrolysate with the strongest antioxidant activity using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) decolouration assay. The optimum conditions obtained from experiments were pH 9.7, 66.2 °C, enzyme concentration = 68.1 Anson units (AU)/kg crude protein. The analysis of variance in RSM showed that pH and temperature (T) were the most important factors of the process (P < 0.001). The experimental conditions produced both an enzymatic and chemical protein solubilization.

Keywords
Alcalase® 2,4L; Antioxidant activity; Shrimp by-product; Hydrolysis; Response surface methodology (RSM)
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Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 11, November 2007, Pages 1486–1491
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
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