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DCO2 on-line measurement used in rapamycin fed-batch fermentation process

Paper ID Volume ID Publish Year Pages File Format Full-Text
35729 45104 2008 5 PDF Available
Title
DCO2 on-line measurement used in rapamycin fed-batch fermentation process
Abstract

A Streptomyces fed-batch fermentation was studied with on-line measurement of dissolved carbon dioxide (DCO2). A selected cell strain R060107 of Streptomyces hygroscopicus was used in the production of rapamycin. pH was controlled only in the production phase at the low limit of 5.1 after its value dropped from an initial 6.5 to 5.1. DCO2 was used to control the feed in the fed-batch fermentation of S. hygroscopicus R060107. The experiment investigated the relationship between rapamycin production and the evolution of DCO2. A high limit of DCO2 (hDCO2) was used to control medium feed. In order to meet the needs for the dynamic growth and metabolism of cells in the process, the hDCO2 was defined as a dynamic parameter, and was therefore calculated constantly in the process. A fermentation condition of limited nutrient supply was realized. It was observed that rapamycin was secreted when the glycerol concentration was well controlled in the fermentation condition. Experimental results showed that 500 mg/l of rapamycin was produced in 120 h of fermentation.

Keywords
DCO2; Streptomyces; Antibiotic; Rapamycin; Fed-batch; Fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 4, April 2008, Pages 351–355
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us