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Ligninolytic enzyme ability and potential biotechnology applications of the white-rot fungus Grammothele subargentea LPSC no. 436 strain

Paper ID Volume ID Publish Year Pages File Format Full-Text
35732 45104 2008 8 PDF Available
Title
Ligninolytic enzyme ability and potential biotechnology applications of the white-rot fungus Grammothele subargentea LPSC no. 436 strain
Abstract

To get a better insight into the ligninolytic system of Grammothele subargentea, extracellular ligninolytic enzyme activities and ability to degrade synthetic dyes as well as Eucalyptus globulus wood were assayed in cultures grown on an agar medium with Cu2+ or dyes and on E. globulus wood chips. Laccase was the only ligninolytic enzyme detected. The fungus was able to decolorize different dyes, being the highest levels of laccase activity in cultures with Brilliant Green. Cultures on wood showed both ligninolytic activity and degradative ability on lipophilic extractives. An extracellular laccase with pI 3.5 and maximal activity at pH 4.0 and 50–55 °C was detected on liquid cultures containing 0.6 mM Cu2+. The enzyme extract was stable at pH 6.0–7.0 and up to 60 °C. A laccase-mediator system using a G. subargentea laccase crude extract and 1-hydroxybenzotriazole as mediator improved the tensile strength of a paper from recycled high-kappa-number pulp.

Keywords
Dye decolorization; Extracellular ligninolytic enzymes; Laccase; Paper industry; White-rot fungus; Wood degradation
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Ligninolytic enzyme ability and potential biotechnology applications of the white-rot fungus Grammothele subargentea LPSC no. 436 strain
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 4, April 2008, Pages 368–375
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us