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Medium optimization for biomass production and morphology variance overcome of Photorhabdus temperata ssp. temperata strain K122

Paper ID Volume ID Publish Year Pages File Format Full-Text
35747 45105 2008 7 PDF Available
Title
Medium optimization for biomass production and morphology variance overcome of Photorhabdus temperata ssp. temperata strain K122
Abstract

Photorhabdus temperata ssp. temperata strain K122 is an important pathogen of the olive moth, Prays oleae. A new medium containing 5 g/l glucose, 10 g/l yeast extract and 1.07 g/l ammonium chloride is presented that optimizes the biomass production of strain K122. We also showed for the first time that P. temperata ssp. temperata strain K122 develops variants small colony (Vsm) polymorphism after prolonged incubation. Instability and reversion from one form to another are described. Since Vsm cells reduce pathogenicity against insect larvae, conditions to avoid their appearance were determined. Medium aeration and inoculum quality are demonstrated as key factors to reduce Vsm occurrence. Maintenance of initial glucose concentration during incubation not only doubled biomass production, but also contributed to complete overcome of Vsm appearance. Following oral application, it was shown that P. temperata ssp. temperata cells produced at such conditions continued to exhibit their toxicity against Ephestia kuehniella. This study might contribute to promote the large-scale use of P. temperata ssp. temperata as a bioinsecticide.

Keywords
P, Photorhabdus; Vsm, variants small colony; E, Ephestia; P, Prays; DNA, deoxyribonucleic acid; 16S rDNA, 16S ribosomal DNA; EDTA, ethylene diamine tetraacetic acid; PCR, polymerase chain reaction; CTAB, cetyltrimethyl ammonium bromidePhotorhabdus tempera
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Medium optimization for biomass production and morphology variance overcome of Photorhabdus temperata ssp. temperata strain K122
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 12, December 2008, Pages 1338–1344
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us