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Production of 3-hydroxypropionic acid from glycerol by a novel recombinant Escherichia coli BL21 strain

Paper ID Volume ID Publish Year Pages File Format Full-Text
35761 45105 2008 7 PDF Available
Title
Production of 3-hydroxypropionic acid from glycerol by a novel recombinant Escherichia coli BL21 strain
Abstract

3-Hydroxypropionic acid (3-HP) is an important platform chemical from which several commodity and specialty chemicals can be generated. The present investigation focuses on the construction and evaluation of a recombinant strain Escherichia coli SH254 that produces 3-HP from glycerol. The strain was developed by cloning two genes, dhaB of Klebsiella pneumoniae DSM 2026 encoding glycerol dehydratase and aldH of E. coli K-12 MG1655 encoding aldehyde dehydrogenase, respectively. In vitro assays of crude enzyme extract of glycerol dehydratase (DhaB) showed 37.0 U mg−1 protein on glycerol with coenzyme B12, and partially purified aldehyde dehydrogenase (AldH) exhibited 22.8 U mg−1 protein on 3-hydroxypropionaldehyde (3-HPA) with NAD+ as a cofactor. When cultivated aerobically on a glycerol medium containing yeast extract, the recombinant E. coli SH254 produced 3-HP at a maximum of 6.5 mmol l−1 (0.58 g l−1). The highest specific rate and yield of 3-HP production were estimated as 6.6 mmol g−1 cdw h−1 and 0.48 mol mol−1 glycerol, respectively. Although not optimized extensively, this study is encouraging for further development of a bioprocess to produce 3-HP from glycerol.

Keywords
Glycerol; 3-Hydroxypropionaldehyde; 3-Hydroxypropionic acid; Recombinant E. coli; Glycerol dehydratase; Aldehyde dehydrogenase
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Production of 3-hydroxypropionic acid from glycerol by a novel recombinant Escherichia coli BL21 strain
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 12, December 2008, Pages 1440–1446
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us