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Production of glutathione in extracellular form by Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
35768 45106 2010 5 PDF Available
Title
Production of glutathione in extracellular form by Saccharomyces cerevisiae
Abstract

The present research was aimed at inducing, in a post fermentative procedure (biotransformation) and by modifying cell permeability, glutathione (GSH) accumulation and subsequent release from cells of Saccharomyces cerevisiae. With the aim of limiting process costs, research considered the possibility of employing baker's yeasts (S. cerevisiae), inexpensive cells source available on the market, in comparison with a collection strain. The tested yeasts showed different sensitivity to the chemical/physical treatments performed to alter cell permeability. Modest effects were evidenced with Triton, active only on Zeus yeast samples (1.7 g GSH/l, near 60% of which in extracellular form). Lauroyl sarcosine showed an interesting action on GB Italy sample (2.8 g GSH/l, near 80% extracellular). Lyophilization evidenced good performance with Lievitalia yeast strain (2.9 g GSH/l, 90% extracellular). The possibility of obtaining GSH directly in extracellular form represents an interesting opportunity of reducing GSH production cost and furthering the range of application of this molecule.

Keywords
Glutathione; Saccharomyces cerevisiae; Extracellular metabolite; Cell permeabilization; Baker's yeast; Extracellular release
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 4, April 2010, Pages 441–445
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us