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Optimization of carbon and nitrogen sources for biomass and lipid production by Chlorella saccharophila under heterotrophic conditions and development of Nile red fluorescence based method for quantification of its neutral lipid content

Paper ID Volume ID Publish Year Pages File Format Full-Text
3577 176 2012 9 PDF Available
Title
Optimization of carbon and nitrogen sources for biomass and lipid production by Chlorella saccharophila under heterotrophic conditions and development of Nile red fluorescence based method for quantification of its neutral lipid content
Abstract

In this study, initially, the carbon and nitrogen source preferences of Chlorella saccharophila were examined in terms of biomass productivities under heterotrophic growth conditions. It was shown that C. saccharophila could actively utilize glucose and glycerol as carbon sources and bacteriological peptone as a nitrogen source. Secondly, the concentrations of nitrogen and carbon sources that were found to significantly influence the biomass productivity of heterotrophic C. saccharophila were further optimized by using Box–Behnken experimental design. Lastly, in a scale-up attempt, the medium consisting of 20 g/L glucose and 1 g/L bacteriological peptone was used in a 3 L stirred tank bioreactor in which the final biomass concentration obtained was 7.7 fold higher than that of obtained under autotrophic conditions. Also, lipid content in heterotrophic cells of C. saccharophila was increased about 3 times compared to that of autotrophic cells. The principal fatty acids in heterotrophic C. saccharophila were oleic acid (C18:1) and linoleic acid (C18:2) constituting 34.4% and 30.1% of the total fatty acid contents, respectively. Moreover, a simple and rapid method determining the neutral lipid accumulation in C. saccharophila with spectrofluorimetry was developed and used easily for monitoring lipid accumulation in a stirred tank bioreactor.

► We study growth, lipid and fatty acid composition of Chlorella saccharophila. ► Higher lipid content has been obtained under heterotrophic cultivation. ► We develop Nile red fluorescence spectrophotometric method. ► The principal fatty acids are oleic and linoleic acids.

Keywords
Microalgae; Heterotrophic culture; Batch processing; Optimization; Bioreactors; Lipid
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Optimization of carbon and nitrogen sources for biomass and lipid production by Chlorella saccharophila under heterotrophic conditions and development of Nile red fluorescence based method for quantification of its neutral lipid content
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 61, 15 February 2012, Pages 11–19
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us