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Reactive oxygen species mediated modifications in Bacillus subtilis lipid membrane to improve protein productivities

Paper ID Volume ID Publish Year Pages File Format Full-Text
35772 45106 2010 8 PDF Available
Title
Reactive oxygen species mediated modifications in Bacillus subtilis lipid membrane to improve protein productivities
Abstract

The overall objective of this work was to investigate the modifications that occur in Bacillus subtilis lipid membrane during induced oxidative stress caused by reactive oxygen species (ROS), via an electrophysiological approach. Further, based on the results, we have developed and demonstrated a novel strategy to enhance specific enzyme production. Electrical parameters such as phase angle (θ), impedance (Z), capacitance (C) and breakdown voltage of reconstituted bilayer lipid membrane (BLM) composed of lipids extracted from non-stressed, mildly stressed (2.5 mM H2O2) and strongly stressed (2.5 mM H2O2 with 100 μM FeSO4) B. subtilis were compared. Strongly stressed BLM showed lower values of θ (10°), Z (0.4 Mohm), and breakdown voltage (100 mV) in comparison with those observed for non-stressed BLM, i.e., 30°, 0.5 Mohm and 250 mV, respectively. The capacitance of strongly stressed BLM, however, was higher (2.28 nF) compared to that of the non-stressed BLM (0.4 nF). These results suggest that under strongly stressed conditions, the lipids were loosely packed that resulted in a more permeable BLM. The higher permeability seems to result, unexpectedly, from a higher unsaturated fatty acid (UFA) synthesis and membrane incorporation (UFA fraction increased by 227%), and expectedly, from increased lipid peroxidation (increased by nearly 200%) in the BLM. A strategy that is based on increased membrane permeability due to induced ROS, enhanced specific amylase and protease production under oxidative stress by 62% and 137%, respectively.

Keywords
Oxidative stress; Reactive oxygen species; Bilayer lipid membrane; Lipid peroxidation; Amylase; Protease
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Reactive oxygen species mediated modifications in Bacillus subtilis lipid membrane to improve protein productivities
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 4, April 2010, Pages 467–474
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us