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A bioprocess for the production of high concentrations of R-(+)-α-terpineol from R-(+)-limonene

Paper ID Volume ID Publish Year Pages File Format Full-Text
35774 45106 2010 6 PDF Available
Title
A bioprocess for the production of high concentrations of R-(+)-α-terpineol from R-(+)-limonene
Abstract

A bioprocess with a high conversion rate of limonene to α-terpineol was described. The enzyme hydratase involved in this process was found as being cofactor independent, non-inducible and able to perform the transformation of both R-(+) and S-(−)-limonene. The system used consisted of a biphasic medium in which the aqueous phase contained a concentrated resting cells of Sphingobium sp. and the organic phase was sunflower oil. After 30 h at 30 °C ca. 25 g of R-(+)-α-terpineol per liter of organic phase were obtained from R-(+)-limonene in Erlenmeyer flasks. Performance of the bioconversion in a bioreactor increased the production rate with no changes in yield and maximal R-(+)-α-terpineol concentration, which demonstrated that experiments in flasks were limited by liquid–liquid transport phenomena. A mathematical model able to explain the fact that the reaction always stopped before the precursor became exhausted has also been proposed and validated. Finally, the process reported was the most promising alternative for the biotechnological production of natural R-(+)-α-terpineol published so far and up to ca. 130 g L−1 metabolite could finally be obtained.

Keywords
Bioflavors; Biphasic media; Vegetable oils; Terpenes; Biotransformation
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A bioprocess for the production of high concentrations of R-(+)-α-terpineol from R-(+)-limonene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 4, April 2010, Pages 481–486
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us