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Preparation of the immobilized metal affinity membrane with high amount of metal ions and protein adsorption efficiencies

Paper ID Volume ID Publish Year Pages File Format Full-Text
35777 45106 2010 7 PDF Available
Title
Preparation of the immobilized metal affinity membrane with high amount of metal ions and protein adsorption efficiencies
Abstract

In this study, an approach to prepare immobilized metal affinity membrane (IMAM) with high metal ions and protein adsorption capacities was developed. In the process of coupling epichlorohydrin (EPI) to the regenerated cellulose membrane (RC membrane), NaOH concentration is found to be the most critical. With a lower NaOH concentration, only a minimal amount of EPI reacted to the RC membrane. When NaOH concentration was higher, the membrane was distorted, which caused a significant pressure drop in flow-through operation. To optimize the IMAM performance, an objective function was defined as the ratio of the model protein, penicillin G acylase (PGA), activity adsorbed on the membrane to the transmembrane pressure drop. According to the criterion, the optimal reaction conditions were found as follows: one RC membrane immersed in 20 ml, 1.4 M NaOH, 5 ml EPI and operated at 24 °C, 150 rpm for 14 h. Under this condition, the copper ions and PGA in IMAM were significantly increased to 75.5 ± 0.25 μmol/disc and 1.8 U/disc respectively. The adsorption for lysozyme on the prepared IMAM reached 1044 μg/cm2, the highest in the literature.

Keywords
Penicillin G acylase; Immobilized metal affinity membrane; Enzymes purification; Optimization; Regenerated cellulose membrane
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Preparation of the immobilized metal affinity membrane with high amount of metal ions and protein adsorption efficiencies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 4, April 2010, Pages 500–506
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us