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Ultrasonic irradiation with vibration for biodiesel production from soybean oil by Novozym 435

Paper ID Volume ID Publish Year Pages File Format Full-Text
35780 45106 2010 7 PDF Available
Title
Ultrasonic irradiation with vibration for biodiesel production from soybean oil by Novozym 435
Abstract

The production of biodiesel with soybean oil and methanol through transesterification by Novozym 435 (Candida antarctica lipase B immobilized on polyacrylic resin) were conducted under two different conditions—ultrasonic irradiation and vibration to compare their overall effects. Compared with vibration, ultrasonic irradiation significantly enhanced the activity of Novozym 435. The reaction rate was further increased under the condition of ultrasonic irradiation with vibration (UIV). Effects of reaction conditions, such as ultrasonic power, water content, organic solvents, ratio of solvent/oil, ratio of methanol/oil, enzyme dosage and temperature on the activity of Novozym 435 were investigated under UIV. Under the optimum conditions (50% of ultrasonic power, 50 rpm vibration, water content of 0.5%, tert-amyl alcohol/oil volume ratio of 1:1, methanol/oil molar ratio of 6:1, 6% Novozym 435 and 40 °C), 96% yield of fatty acid methyl ester (FAME) could be achieved in 4 h. Furthermore, repeated use of Novozym 435 after five cycles showed no obvious loss in enzyme activity, which suggested this enzyme was stable under the UIV condition. These results indicated that UIV was a fast and efficient method for biodiesel production.

Keywords
Ultrasonic irradiation; Vibration; Biodiesel; Soybean oil; Novozym 435
First Page Preview
Ultrasonic irradiation with vibration for biodiesel production from soybean oil by Novozym 435
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 4, April 2010, Pages 519–525
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering