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Stoichiometric balance based macrokinetic model for Penicillium chrysogenum in fed-batch fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
35783 45106 2010 7 PDF Available
Title
Stoichiometric balance based macrokinetic model for Penicillium chrysogenum in fed-batch fermentation
Abstract

A macrokinetic model describing mycelium growth and product formation in fed-batch fermentation of Penicillium chrysogenum is proposed on the basis of intracellular metabolic pathway networks found in the literature. The model is composed of the stoichiometric balance equations for carbon source, ATP, NADH and pyruvate. A regulator model is used as an auxiliary part to simulate the lag phase at the beginning of product synthesis. Combining the macrokinetic model with the bioreactor model, one yields the relationship between manipulating variables, i.e., the substrate feeding rate, and the other state variables such as biomass, substrate and product concentrations. The rolling parameter identification is then applied to online identify the most sensitive model parameters dealing with the model mismatch caused by time-variant kinetic changes. Model validation was performed with the data of 22 batches from industrial-scale penicillin cultivation. Finally, the predictions of the product concentration, up to 24 h ahead, are carried out with an average prediction error of less than 3.5%.

Keywords
Penicillium chrysogenum; Industrial fed-batch fermentation; Macrokinetic model; Validation; Rolling identification-prediction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 4, April 2010, Pages 542–548
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us