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Development of the magnetic beads for dye ligand affinity chromatography and application to magnetically stabilized fluidized bed system

Paper ID Volume ID Publish Year Pages File Format Full-Text
35785 45106 2010 7 PDF Available
Title
Development of the magnetic beads for dye ligand affinity chromatography and application to magnetically stabilized fluidized bed system
Abstract

Magnetic poly(2-hydroxyethylmethacrylate) [mPHEMA] beads were prepared by suspension polymerization of HEMA in the presence of Fe3O4 nano-powder. Cibacron Blue F3GA was covalently immobilized to the mPHEMA beads via nucleophilic substitution reaction between chloride of its triazine ring and hydroxyl groups of HEMA under alkaline conditions. The mPHEMA/Cibacron Blue F3GA beads (100–140 μm in diameter) carrying 68.3 μmol Cibacron Blue F3GA per gram polymer were used for β-casein adsorption studies. Adsorption studies were performed under different conditions in a batch system (i.e., pH, β-casein initial concentration, temperature, and ionic strength) and then in a magnetically stabilized fluidized bed (MSFB) system. The swelling ratio of the mPHEMA was 62.1%. The maximum adsorption capacity for batch system was 20.2% lower as compared to the value obtained in MSFB. The mPHEMA/Cibacron Blue F3GA beads could be repeatedly applied for β-casein adsorption without significant losses in the adsorption capacity.

Keywords
Dye-affinity beads; Magnetic polymers; Protein purification; β-Casein; Magnetic beads
First Page Preview
Development of the magnetic beads for dye ligand affinity chromatography and application to magnetically stabilized fluidized bed system
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 45, Issue 4, April 2010, Pages 556–562
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering