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Isolation and characterization of lectins from kidney beans (Phaseolus vulgaris)

Paper ID Volume ID Publish Year Pages File Format Full-Text
35805 45107 2007 7 PDF Available
Title
Isolation and characterization of lectins from kidney beans (Phaseolus vulgaris)
Abstract

The bioactive properties of lectins obtained from raw and canned red kidney bean (Phaseolus vulgaris) were studied to determine the changes in their bioactivity during the canning process. Phytohaemagglutinin (PHA) was extracted using Affi-gel Blue gel and thyroglobulin-Sepharose and had a molecular weight of 32 kDa. Both the raw and the canned kidney beans possessed the ability to agglutinate red blood cells and inhibit α-glucosidase. The activity found in the canned beans was similar to that from the in the raw kidney beans. However, the amount of lectin that could be extracted from thyroglobulin-Sepharose was much less in the canned samples than in the raw kidney bean samples. The extracted lectin from the raw kidney beans was also subjected to a heating and cooling treatment using a differential scanning calorimeter. The lectin had a nonset denaturation temperature of 77.76 °C and it did not renature upon cooling. In this study, we demonstrated that extracts from raw red kidney bean and canned red kidney bean contain bioactive compounds capable of inhibiting HIV-1 RT in vitro.

Keywords
Lectin; HIV; Haemagglutination; Kidney bean; Bioactivity property
First Page Preview
Isolation and characterization of lectins from kidney beans (Phaseolus vulgaris)
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 10, October 2007, Pages 1436–1442
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering