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Production of (R)-mandelic acid by immobilized cells of Saccharomyces cerevisiae on chitosan carrier

Paper ID Volume ID Publish Year Pages File Format Full-Text
35810 45107 2007 5 PDF Available
Title
Production of (R)-mandelic acid by immobilized cells of Saccharomyces cerevisiae on chitosan carrier
Abstract

The asymmetric microbial reduction of phenylglyoxylic acid (PGA) to (R)-mandelic acid ((R)-MA) with immobilized Saccharomyces cerevisiae cells on globular chitosan was studied. The immobilization conditions and characterization of the immobilized cells were carried out. Chitosan–acetic acid solution was injected into a mixture of 20% NaOH and 30% CH3OH aqueous solution to obtain globular chitosan, and then the globular chitosan was treated with 1% solution of glutaraldehyde to immobilize yeast cells, which were used to synthesize (R)-MA. The optimum conditions were identified as the substrate concentration of 10 mmol L−1, pH of 6.5 and reaction temperature of 30 °C with the yield of 62% for (R)-MA and the enantiomeric excess (e.e.) of 98% for (R)-MA. The immobilized cells showed good operation and storage stability.

Keywords
Saccharomyces cerevisiae; Globular chitosan; Immobilized cells; Asymmetric reduction; Phenylglyoxylic acid; (R)-Mandelic acid
First Page Preview
Production of (R)-mandelic acid by immobilized cells of Saccharomyces cerevisiae on chitosan carrier
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 10, October 2007, Pages 1465–1469
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering