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Production of demethylated colchicine through microbial transformation and scale-up process development

Paper ID Volume ID Publish Year Pages File Format Full-Text
35831 45109 2008 7 PDF Available
Title
Production of demethylated colchicine through microbial transformation and scale-up process development
Abstract

Mutant strain of Bacillus megaterium ACBT03 is capable of demethylation at third carbon position of colchicine and their derivatives. A wild strain of B. megaterium ACBT03 collected from field was grown at shake flask level and the most suitable sources of carbon and nitrogen were studied in order to increase the biotransformation process. Glucose in combination with glycerol having 2:1 ratio, and yeast extract plus peptone 1:3 ratio, at pH 7.0 and 28 °C incubation temperature were noticed to be the most suitable conditions for maximum biotransformation. The potential of demethylation of the wild strain was noticed to be very poor (20–25% of substrate supplied) when 0.1 g/l colchicine or thiocolchicine was used. In order to increase the potential for demethylation, B. megaterium ACBT03 was subjected to enrichment culture with higher concentration of colchicine. The bacterial cells were grown for 8–10 generations, in higher concentration of colchicine. The newly mutant developed through colchicine enrichment culture was named as B. megaterium ACBT03-M3. About 55% of colchicine and 60% of thiocolchicine were converted to their respective demethylation form, when this mutant was grown in 7 g/l colchicine and thiocolchicine, both at laboratory-scale (5-l-jar fermenter) and pilot-scale level (70-l fermenter). Under optimum culture conditions the key monitoring factors to scale-up the process of demethylation were dissolved oxygen (DO) level (2.5 vvm) of culture broth and impeller tip velocity (4710 cm/min).

Keywords
Microbial transformation; Scale-up; Colchicine; Thiocolchicine; Fermenter; 3-Demethylated colchicine; 3-Demethylated thiocolchicine; Bacillus spp
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 3, March 2008, Pages 251–257
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us