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Stabilization of an enzymatic extract from Penicillium camemberti containing lipoxygenase and hydroperoxide lyase activities

Paper ID Volume ID Publish Year Pages File Format Full-Text
35832 45109 2008 7 PDF Available
Title
Stabilization of an enzymatic extract from Penicillium camemberti containing lipoxygenase and hydroperoxide lyase activities
Abstract

The stabilization of an enzymatic extract, obtained from Penicillium camemberti containing lipoxygenase (LOX) and hydroperoxide lyase (HPL) activities, was investigated using selected additives. Although the addition of KCl (86%, w/w) to the enzymatic extract decreased slightly (7%) the LOX activity, it increased HPL activity by 2.25 fold; however, the addition of dextran resulted in the inactivation of both enzymatic activities. The stability of the solid lyophilized enzymatic extract was greater in the presence of KCl than that without it, with ∼100% residual activity after 8 and 4 weeks of storage at −80 °C, for LOX and HPL, respectively. The rate constants of inactivation (Kinactivation) and the C(1/2) values showed that glycine was the most appropriate additive for LOX and HPL. All other investigated additives, including glycerol, polyethylene glycol, mannitol and sucrose, demonstrated a higher inactivation effect on both enzymatic activities. Although most of the investigated additives provided certain enhancement of the thermostability of LOX activity but not for HPL, a high thermostability (80 °C, 1 h) was obtained with 5% mannitol for LOX and 10% glycine for HPL.

Keywords
Penicillium camemberti; Lipoxygenase; Hydroperoxide lyase; Additives; Stabilization
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Stabilization of an enzymatic extract from Penicillium camemberti containing lipoxygenase and hydroperoxide lyase activities
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 3, March 2008, Pages 258–264
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us