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Glucose oxidase immobilization by polyurethane film/foam in dense CO2 environment

Paper ID Volume ID Publish Year Pages File Format Full-Text
35834 45109 2008 9 PDF Available
Title
Glucose oxidase immobilization by polyurethane film/foam in dense CO2 environment
Abstract

Glucose oxidase (GOX) is immobilized by two different polymer matrices (polyurethane (PU) film and foam) in supercritical carbon dioxide (scCO2) environment for an envisioned application in biosensor preparations. Effects of immobilization temperature (25–50 °C), pressure (0.1–15 MPa) and immobilization time (4–72 h) on the GOX activity of immobilized PU samples are studied. The interactions between PU and CO2 are also experimentally investigated. Maximum surface area enlargements during foaming between 12 and 250% of the original for PU films and almost no swelling/foaming (0.3–4.4% of original) are observed for PU foams. The swelling/foaming characteristics of PU films are positively correlated with the observed pressure effect on GOX immobilized sample activities. Immobilized sample activities are found to be inversely proportional to the immobilization temperature. Activities obtained for samples prepared at 8, 15 and especially at 10 MPa are 52, 74 and 165%, respectively, greater than the highest value obtained for samples immobilized without using the supercritical CO2 process.

Keywords
Supercritical carbon dioxide; Enzyme; Glucose oxidase; Polyurethane; Immobilization; Biosensor
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Glucose oxidase immobilization by polyurethane film/foam in dense CO2 environment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 3, March 2008, Pages 271–279
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us