fulltext.study @t Gmail

Consecutive microwave irradiation induced substrate inhibition on the enzymatic esterification

Paper ID Volume ID Publish Year Pages File Format Full-Text
35838 45109 2008 5 PDF Available
Title
Consecutive microwave irradiation induced substrate inhibition on the enzymatic esterification
Abstract

The effect of consecutive microwave irradiation on the enzymatic esterification of pentanol with caprylic acid was investigated. Lipase from Mucor miehei was used as the catalyst under consecutive microwave irradiation and conventional heating, respectively. Microwave irradiation was found to increase the enzyme affinity to pentanol, which has not been reported in the literature. The conventional heating reaction conducted at an initial concentration of caprylic acid lower than 0.25 mol/L was found to fit the Ping-Pong Bi-Bi mechanism better than the microwave-assisted reaction. The microwave-assisted esterification departed from the Ping-Pong Bi-Bi mechanism independent of the initial caprylic acid concentration when the caprylic acid concentration was 0.25 mol/L and above. In addition, a pentanol inhibition under microwave irradiation was observed if the pentanol concentration was higher than 0.75 mol/L and caprylic acid concentration was over 0.25 mol/L. The pentanol inhibition caused by the microwave irradiation did not happen in the conventional heating reaction. This phenomenon has not previously been reported in the literature. The experimental results are correlated to the polarity of the substrates. These findings will benefit the understanding of nonthermal effect of microwave irradiation.

Keywords
Consecutive microwave; Nonthermal effect; Esterification; Enzymatic; Inhibition; The Ping-Pong Bi–Bi mechanism
First Page Preview
Consecutive microwave irradiation induced substrate inhibition on the enzymatic esterification
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 3, March 2008, Pages 306–310
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us