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Simulation of ex vivo bone marrow culture: Application to chronic myeloid leukaemia growth model

Paper ID Volume ID Publish Year Pages File Format Full-Text
3584 176 2012 12 PDF Available
Title
Simulation of ex vivo bone marrow culture: Application to chronic myeloid leukaemia growth model
Abstract

The three-dimensional (3-D) architecture of the bone marrow (BM) supports the renewal, maintenance, proliferation and differentiation of haematopoietic stem cells (HSCs). Efforts have been made towards the reconstruction of an ex vivo HSC culture system, which could have several potential applications, such as the detailed understanding of normal and leukaemic haematopoiesis and expansion of haematopoietic stem cells for use in bone marrow transplantation (BMT). In this paper, a numerical model has been developed to investigate the advanced aspects of haematopoiesis, the regulatory effects of growth factor granulocyte colony-stimulating factor (G-CSF), thrombopoietin (TPO) and erythropoietin (EPO) on the production of chronic myeloid leukaemia (CML) cells within a purpose-designed scaffold placed inside a perfused bioreactor. A multi-lineage cellular growth model of haematopoietic cells was incorporated in order to mimic the complexity of the diseased BM. The simulation results demonstrate that different combinations and concentrations of cytokines can alter the self-renewal, differentiation, and proliferation of CML cells and this effect is also dependent upon operation conditions and scaffold properties. The numerical model presented here enables the optimisation of scaffold design parameters and mass transfer environment necessary for developing a suitable ex vivo haematopoietic system.

► Development of a numerical model to investigate the advanced aspects of haematopoiesis. ► Regulatory effects of growth factors on the production of chronic myeloid leukaemia cells. ► Purpose-designed scaffold placed inside a perfused bioreactor.

Keywords
Computational fluid dynamics; Chronic myeloid leukaemia; Bioreactors; Growth kinetics and mass transfer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 61, 15 February 2012, Pages 66–77
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us