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Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus

Paper ID Volume ID Publish Year Pages File Format Full-Text
35846 45110 2008 8 PDF Available
Title
Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus
Abstract

Termitomyces clypeatus produces cellobiose dehydrogenase (CDHtc) in cellulose medium with the highest yield (55.88 U mL−1) among all the reported fungal species. The enzyme has been isolated and purified from the culture filtrate.CDHtc was found to be a very thermolabile enzyme with the temperature optimum at 30 °C, while it exhibited a wide range of pH stability from pH 2.0 to 8.0. Lactose was efficiently converted to lactobionic acid in presence of the enzyme. Addition of glucose in the cellulose medium on the first day of growth induced a lag period in enzyme production but ultimately facilitated earlier CDHtc production and the yield was also comparable to that achieved in the cellulose medium.

Keywords
Cellobiose dehydrogenase; Termitomyces clypeatus; Edible fungus; Thermolabile enzyme; Lactobionic acid; Glucose effect
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 6, June 2008, Pages 634–641
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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