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Enhanced intracellular accumulation of recombinant HBsAg in CHO cells by dimethyl sulfoxide

Paper ID Volume ID Publish Year Pages File Format Full-Text
35854 45110 2008 6 PDF Available
Title
Enhanced intracellular accumulation of recombinant HBsAg in CHO cells by dimethyl sulfoxide
Abstract

The intracellular hepatitis B surface antigen (HBsAg) content per cell was significantly increased by 7.2-fold in the culture of recombinant CHO cells with 1.5% dimethyl sulfoxide (DMSO), while the HBsAg production and specific productivity were only improved by 70% and 3.2-fold, respectively. The significant accumulation of HBsAg within rCHO cells by DMSO stimulation was testified with flow cytometry measurements. Electron microscopy was applied to show that the dilated areas scattered over whole cytoplasm within rCHO cells in response to DMSO, and further revealed that intracellular HBsAg was localized to these areas with immunogold labeling. The failure of intracellular HBsAg virus-like particle assembly was revealed to be closely associated with the HBsAg accumulation within DMSO-stimulated rCHO cells on the basis of sucrose gradient analysis of cell extract. This work provided the details to further understand the HBsAg accumulation within rCHO cells in response to DMSO.

Keywords
Cell culture; Recombinant CHO cell; Dimethyl sulfoxide; HBsAg; Sucrose gradient analysis; Intracellular accumulation
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Enhanced intracellular accumulation of recombinant HBsAg in CHO cells by dimethyl sulfoxide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 6, June 2008, Pages 690–695
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
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