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Demineralization of crab shell waste by Pseudomonas aeruginosa F722

Paper ID Volume ID Publish Year Pages File Format Full-Text
35861 45111 2007 6 PDF Available
Title
Demineralization of crab shell waste by Pseudomonas aeruginosa F722
Abstract

Crab shell (CS) waste samples (particle size 3–10 and 20–35 mm) were inoculated with the newly isolated Pseudomonas aeruginosa F722 to study the efficiency of microbial demineralization (DM) and deproteinization (DP) in the process of extracting chitin. The inoculated waste was incubated for 7 days at 25, 30 and 35 °C. Various concentrations of glucose were supplemented as carbon source. At the optimal temperature of 30 °C, DM was 92% and DP was 63% DP, whereas the pH dropped from initial pH 8.0 to 4.1. In comparative experiments with different amounts of CS waste, 5% CS waste treatment was shown to be the optimal amount for efficient DM. A positive relationship is correlated between DM and glucose concentration (r2 = 0.821), whereas a negative relationship is correlated between DM and pH (r2 = 0.793). DP and protease activity were little affected by different crab shell sizes.

Keywords
Pseudomonas aeruginosa F722; Crab shell waste; Demineralization; Deproteinization; Glucose supplement
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 7, July 2007, Pages 1069–1074
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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