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Coupling oligo-β-cyclodextrin on polyacrylate beads media for separation of puerarin

Paper ID Volume ID Publish Year Pages File Format Full-Text
35862 45111 2007 9 PDF Available
Title
Coupling oligo-β-cyclodextrin on polyacrylate beads media for separation of puerarin
Abstract

The isoflavonoid puerarin, a well-known bioactive constituent in traditional Chinese medicine, can be separated from other isoflavonoids in the extracts of Radix puerariae (root of the plant Pueraria lobata) on oligo-β-cyclodextrin ligand coupled to D152 beads. The parameters of coupling time, temperature, solvent system and molar ratio of reactants were tested to optimize the preparation conditions. Acetic acid (7%) was used as the optimum mobile phase. The fine particle sizes of the coupled microspheres packing displayed high resolving power and efficiency. Compared with other two polyacrylate carrier media, PGMA-CDP and PHEMA-CDP, various retention mechanisms such as β-CD inclusion complexation, hydrogen bond interaction, and π–π interaction might exist in the chromatography process. This study provides a novel alternative matrix for the separation of puerarin.

Keywords
β-Cyclodextrin; Chromatography; Polyacrylate beads media; Coupling; Separation; Puerarin
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Coupling oligo-β-cyclodextrin on polyacrylate beads media for separation of puerarin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 7, July 2007, Pages 1075–1083
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us